Basophil degranulation test

Specific allergy diagnostics using leukotriene detection

The determination of allergen-specific IgE is a widespread and well-established method of in vitro allergy diagnostics. In certain cases, such as pseudo-allergies of the immediate reaction type that do not lead to any increase in specific IgE, the basophil degranulation test (BDT) represents an important supplement to laboratory diagnostics and is often the only meaningful diagnostic method possible. This also applies to commercially available allergen testing methods and to the testing of native allergens.

Diagnostics
Basophil granulocytes and mast cells are the primary effector cells of allergic reactions such as rhinitis, asthma, urticaria and anaphylactic shock. After specific stimulation with an allergen, the cells produce sulfidoleukotrienes. These inflammation mediators are detected using an enzyme immunoassay. In this respect, BDT is an in vitro stimulation of the reaction taking place in vivo. In many cases, BDT makes it possible to dispense with a provocation test. This is a great advantage, especially in paediatrics.

Indication

  • Requesting a BDT is advisable for the following indications:
  • For difficult allergens such as insect venoms, medicines, food additives, latex, medical dental materials like acrylates and other native materials
  • For IgE-negative patients with allergic symptoms
  • For pseudo-allergies of the immediate reaction type
  • When testing for native allergens (in addition to the standard allergens mentioned on the request form, native preparations can be tested as well; if so, please also include ampoules or trays with the blood being sent to the laboratory)
  • If the skin test has to be omitted because of the danger of an anaphylactic shock

Test material
A separate EDTA Monovette is required for BDT.

  • If necessary, the suspected allergen
  • 2 ml of EDTA blood for individual tests
  • 4 ml of EDTA blood for group tests
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